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Heidenhain's stains

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Also known as Heidenhains Färbung (German).

Heidenhain's stain 1: Eisenhämatoxylin nach Heidenhain (German), Heidenhain's iron alum haematoxylin stain. Iron alum haematoxylin stain. Muscle striations and mitotic structures are rendered blue-black.

Heidenhain's stain 2: Azanfärbung nach Heidenhain German), Heidenhain's azan stain. Heidenhains Azanfärbung (German): Azocarmine and aniline blue-golden orange. Nuclei and cytoplasm rendered red, glia fibrils reddish, mucin blue, collagenic and reticular fibres as well as mucus become dark blue.

Bibliography

  • M. Heidenhain:
    Eine neue Verwendung des Hämatoxylin.
    Archiv für mikroskopische Anatomie, 1885, 24: 468-470. Eine Abänderung der Färbung mit Hämatoxylin und chromosauren Salzen.
    Archiv für mikroskopische Anatomie, 1886, 27: 383-384.

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